Level 3

When gene targeting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as:

Fill in the blanks with appropriate options:

(i) __$\mathrm{A}$__ is removed during the processing of proinsulin to insulin.

(ii) The exploitation of bioresources without proper authorization is called __$\mathrm{B}$__.

Read the following statements:

(i) Agrobacterium tumefaciens infects the roots of the tobacco plant.
(ii) Enzymes present in the saliva of insects convert the inactive form of Bt toxin into an active form.
(iii) Bt corn has been made resistant to corn borer by introducing the crylAc gene.
(iv) Foods derived from transgenic crops are called GM foods.

Which of these statements are correct?

Which of the following is a permanent cure for ADA deficiency?

Some steps involved in the production of genetically engineered insulin are given below. Choose the correct sequence.

(i) Isolation of the insulin gene from a cell.

(ii) Culturing recombinant bacteria in the medium.

(iii) Insertion of the human insulin gene into a plasmid.

(iv) Introduction of the recombinant plasmid into bacteria.

(v) Production of active insulin.

(vi) Extraction of a recombinant gene product from the culture medium.

You discovered a novel eukaryotic organism that glows in the dark. You believe this trait is due to a single gene, and you wish to clone the gene. Which of the following strategies is most likely to be successful?

With regard to insulin choose correct options.

(a) C-peptide is not present in mature insulin.

(b) The insulin produced by rDNA technology has C-peptide.

(c) The pro-insulin has C-peptide.

(d) A-peptide and B-peptide of insulin are interconnected by disulphide bridges.

Match Column I with Column II and select the correct option.