Embibe Experts Solutions for Chapter: Principles and Processes of Biotechnology, Exercise 1: TS EAMCET - 10 August 2021 Shift 1

Study the following lists:

The correct match is

Match the following lists:

The correct match is

In a restriction enzyme digestion experiment, a $\mathrm{DNA}$ fragment of $1$ kilobases length is used. This fragment harbors three $5^{\text{'}}-\mathrm{GAATTC}-3^{\text{'}}$ restriction sites. After the restriction digestion of this fragment with EcoRI, $3 \mathrm{DNA}$ fragments corresponding to $500, 300 \& 100$ base pairs are observed in the agarose gel electrophoresis $\left(\mathrm{AGE}\right)$. Predict the positions of the restriction site $\left(\mathrm{GAATTC}\right)$ in the undigested $1 \mathrm{kb}$-fragment which will result in the observed bands in $\mathrm{AGE}$.

Match the following lists.

The correct match is:

Features of sparged stirred tank bioreactor that differ from simple stirred bioreactors
I) More bubble formation
II) Decrease the surface area for ${\mathrm{O}}_2$ transfer
III) It has foam control system

Which DNA fragment of a vector that helps in identifying and eliminating non-transformant cells and permit the growth of transformant cells?

Assertion (A): Chromogenic substrates give blue-coloured colonies if the plasmid does not have an insert.

Reason (R): Recombinant colonies produce colour in presence of the chromogenic substrates due to the presence of $\mathrm{\beta }$ galactosidase.
The correct option among the following is:

Identify the correct statements.
(A) In gel electrophoresis, DNA fragments resolve according to their size.
(B) It is not necessary to digest the bacterial cells with lysozyme to isolate DNA
(C) In PCR, DNA polymerase extends the primers using the nucleotides.
(D) During the isolation of DNA, ribonuclease is added along with protease
(E) Agarose gel electrophoresis is employed to check the progression of restriction enzyme digestion.