Subject Experts Solutions for Exercise 1: MCQS CORNER

Repressive operon system is usually found in     (i)     pathways.
The pathway's end product serves as a     (ii)     to activate the repressor, turn off enzyme synthesis and prevent overproduction of the end product of the pathway.
Genes for this operon are usually switched     (iii)     and the repressor is synthesised in an     (iv)     form.

Match Column l with Column II and select the correct option from the given options.

Human genome consists of approximately

Estimated number of genes in human beings is ------------.

Find the incorrect statement regarding 'human genome'.

Select the correct option that correctly fill the blanks i-iv.
i. less than $\underset{\_}{\mathrm{ }\left(\mathrm{i}\right)\mathrm{ }}$ of genome represents structural genes that option for proteins.
ii. Chemical substance that binds with repressor and convert it into a non-DNA binding state is $\underset{\_}{\mathrm{ }\left(\mathrm{i}\mathrm{i}\right)\mathrm{ }}$ .
iii. In prokaryotes, during replication RNA primer is removed by $\underset{\_}{\mathrm{ }\left(\mathrm{i}\mathrm{i}\mathrm{i}\right)\mathrm{ }}$ whereas in eukaryotes it is removed by $\underset{\_}{\mathrm{ }\left(\mathrm{i}\mathrm{v}\right)\mathrm{ }}$ .

Arrange the various steps of DNA fingerprinting technique in the correct order.
(i) Separation of DNA fragments by electrophoresis.
(ii) Digestion of DNA by restriction endonucleases.
(iii) Hybridisation using labelled VNTR probe.
(iv) Isolation of DNA.
(v) Detection of hybridised DNA fragments by auto-radiography.
(vi) Transferring the separated DNA fragments to nitrocellulose membrane.

Match Column I with Column II and select the correct option from the given options.