Sergey Bylikin, Gary Horner and, Brian Murphy Solutions for Chapter: Biochemistry, Exercise 4: Questions

The given figure represents a thin layer chromatogram of an amino acid. 

Outline the principle of thin layer chromatography. Refer in your answer to the nature of the mobile and stationary phases.

State one advantage of thin layer chromatography over paper chromatography.

Calculate the ${\mathrm{R}}_{\mathrm{f}}$ of the amino acid if a chromatogram shows a spot at a distance of $40 \mathrm{mm}$ from the start line, and the distance between the start line and the solvent front is $46 \mathrm{mm}$.

Describe how locating agents are used in paper chromatography and gel electrophoresis?

Discuss the differences between a traditional catalyst and an enzyme.

At a very low concentration of a certain substrate, the rate of the enzyme-catalysed reaction doubles, when the substrate concentration increases two times. Explain whether the same effect would be observed at a very high substrate concentration.

Enzymes are protein molecules that catalyse specific biochemical reactions. The phosphorylation of glucose is the first step of glycolysis (the oxidation of glucose) and is catalysed by the enzyme hexokinase. Explain how enzymes such as hexokinase are able to catalyse reactions?

State and explain the effect of increasing temperature from ${20}^{°} \mathrm{C}$ to ${60}^{°} \mathrm{C}$ on an enzyme-catalysed reaction.