RoseMarie Gallagher and Paul Ingram Solutions for Chapter: Separating Substances, Exercise 5: Q

Explain in your own words how paper chromatography works.

What do you think a locating agent is? 

Which chemical you will need, in an experiment to separate amino acids by chromatography?

What makes ${\mathrm{R}}_{\mathrm{f}}$values so useful?

For the chromatogram above:

Were any of the amino acids in B-E also present in A? How can you tell at a glance? 

For the chromatogram above:

(solvent front travelled= $3.5$)

The amino acids travelled the distance from A-E is given, work out the ${\mathrm{R}}_{\mathrm{f}}$values for the amino acids in A-E.

Use the ${\mathrm{R}}_{\mathrm{f}}$table above to name the amino acids. (The Rf values of some amino acids are given)