Analyse the given diagram which shows steps involved in the procedure of selecting transformed bacteria.

 

Identify the bacterial colony which has undergone transformation?

The nucleic acid extracted from the animal liver is loaded and run on an agarose gel. After staining, it shows the following pattern:

If the remaining sample is treated with $\mathrm{RNAse}$ and loaded in gel what result would you expect?

The basic procedure involved in the synthesis of recombinant $\mathrm{DNA}$ molecule is depicted below.

The mistake in the procedure is: