MEDIUM
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Correct statement about restriction endonuclease.

(I) It is the enzyme which cuts DNA at a particular point.
(II) The first restriction endonuclease was HindII.
(III) Overall, 900 restriction enzymes have been isolated from about 200 strains.
(IV) Each restriction endonuclease recognizes a specific palindromic sequence.

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Important Questions on Biotechnology - Principles and Processes

EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The cutting of DNA at a specific location became possible with the discovery of:
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which of the following is a restriction endonuclease?
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which one of the following represents a palindromic sequence in DNA?
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
What is the following is correct for the movement of DNA fragments on agarose gel during gel electrophoresis?
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Commonly used vectors for human genome sequencing are:
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
A gene whose expression helps to identify transformed cell is known as
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
In gel electrophoresis, separated DNA fragments can be visualized with the help of
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The DNA fragments separated on an agarose gel can be visualised after staining with:
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
109 bacteria were spread on an agar plate containing penicillin. After incubation overnight at 37oC 10 bacterial colonies were observed on the plate. That the colonies are likely to be resistant to penicillin can be tested by
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
DNA's precipitation out of a mixture of biomolecules can be achieved by treatment with:
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which vector can clone only a small fragment of DNA?
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Following statements describe the characteristics of restriction endonucleases. Identify the incorrect statement.

EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Identify the palindromic sequence.
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The specific palindromic sequence which is recognized by EcoRl is
MEDIUM
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Identify the wrong statement with regard to restriction enzymes.
HARD
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Eco RI and Rsa I restriction endonucleases require 6bp and 4bp sequences respectively for cleavage. In a 10 kb DNA fragment how many probable cleavage sites are present for these enzymes
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Restriction enzyme work by cleaving
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The introduction of T-DNA into plants involves :