HARD
Earn 100

Define cloning sites. Also show cloning sites of any vector with example.

Important Questions on Biotechnology : Principles and Processes

EASY
Biology>Biotechnology>Biotechnology : Principles and Processes>Tools of Recombinant DNA Technology
What is the following is correct for the movement of DNA fragments on agarose gel during gel electrophoresis?
EASY
Biology>Biotechnology>Biotechnology : Principles and Processes>Tools of Recombinant DNA Technology
Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?
EASY
Biology>Biotechnology>Biotechnology : Principles and Processes>Tools of Recombinant DNA Technology
The DNA fragments separated on an agarose gel can be visualised after staining with:
EASY
Biology>Biotechnology>Biotechnology : Principles and Processes>Tools of Recombinant DNA Technology
109 bacteria were spread on an agar plate containing penicillin. After incubation overnight at 37oC 10 bacterial colonies were observed on the plate. That the colonies are likely to be resistant to penicillin can be tested by
EASY
Biology>Biotechnology>Biotechnology : Principles and Processes>Tools of Recombinant DNA Technology
The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of
HARD
Biology>Biotechnology>Biotechnology : Principles and Processes>Tools of Recombinant DNA Technology
A scientist has cloned an 8 Kb fragment of a mouse gene into the Eco RI site of a vector of 6 Kb size. The cloned DNA has no other Eco RI site within. Digestions of the cloned DNA is shown below

Question Image

Which one of the following sets of DNA fragments generated by digestion with both Eco RI and Bam HI as shown in (iii) is from the gene?
EASY
Biology>Biotechnology>Biotechnology : Principles and Processes>Tools of Recombinant DNA Technology
Genomic DNA is digested with Alu I, a restriction enzyme which is a four base-pair cutter. What is the frequency with which it will cut the DNA assuming a random distribution of bases in the genome?
EASY
Biology>Biotechnology>Biotechnology : Principles and Processes>Tools of Recombinant DNA Technology
How many linear DNA fragments will be produced when a circular plasmid is digested with a restriction enzyme having 3 sites?
EASY
Biology>Biotechnology>Biotechnology : Principles and Processes>Tools of Recombinant DNA Technology
The cutting of DNA at a specific location became possible with the discovery of:
EASY
Biology>Biotechnology>Biotechnology : Principles and Processes>Tools of Recombinant DNA Technology
Restriction enzyme work by cleaving
EASY
Biology>Biotechnology>Biotechnology : Principles and Processes>Tools of Recombinant DNA Technology
A gene whose expression helps to identify transformed cell is known as
EASY
Biology>Biotechnology>Biotechnology : Principles and Processes>Tools of Recombinant DNA Technology
Which of the following is a restriction endonuclease?
MEDIUM
Biology>Biotechnology>Biotechnology : Principles and Processes>Tools of Recombinant DNA Technology

Consider the linear double-stranded DNA shown below. The restriction enzyme sites and the lengths demarcated are shown. This DNA is completely digested with both Eco RI and Bam HI restriction enzymes. If the product is analyzed by gel electrophoresis, how many distinct bands would be observed?

Question Image

HARD
Biology>Biotechnology>Biotechnology : Principles and Processes>Tools of Recombinant DNA Technology
Eco RI and Rsa I restriction endonucleases require 6bp and 4bp sequences respectively for cleavage. In a 10 kb DNA fragment how many probable cleavage sites are present for these enzymes
EASY
Biology>Biotechnology>Biotechnology : Principles and Processes>Tools of Recombinant DNA Technology
In biotechnology applications, a selectable marker is incorporated in a plasmid
EASY
Biology>Biotechnology>Biotechnology : Principles and Processes>Tools of Recombinant DNA Technology
Which one of the following represents a palindromic sequence in DNA?
HARD
Biology>Biotechnology>Biotechnology : Principles and Processes>Tools of Recombinant DNA Technology
A circular plasmid of 10,000 base pairs (bp) is digested with two restriction enzymes, A and B, to produce 3000 bp and 2000 bp bands when visualised on an agarose gel. When digested with one enzyme at a time, only one band is visible at 5000 bp. If the first site for enzyme A (A1) is present at the 100th base, the order in which the remaining sites (A2, B1, and B2) are present is:
EASY
Biology>Biotechnology>Biotechnology : Principles and Processes>Tools of Recombinant DNA Technology
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
EASY
Biology>Biotechnology>Biotechnology : Principles and Processes>Tools of Recombinant DNA Technology
The introduction of T-DNA into plants involves :
EASY
Biology>Biotechnology>Biotechnology : Principles and Processes>Tools of Recombinant DNA Technology
Which of the following is not a feature of the plasmids?