Write the types of restriction enzyme. "Synthesis of recombinant DNA molecule is possible only when the vector and source DNA is cut by the same restriction enzyme" explain reason.

The nuclease enzymes cleave the nucleic acids. There are two types of restriction enzymes: exonuclease and endonuclease. The exonuclease cleaves the DNA strands from either of the two ends and the endonuclease cleaves the DNA at any point other than the two ends i.e. they cut the DNA from within. There are three types of restriction endonuclease enzymes that are used in biotechnology. These are Type I, Type II and Type III. The commonly used enzymes in biotechnology are the restriction endonuclease enzymes Type II which possess a separate nuclease and methylase activity. Type II restriction endonucleases are components of restriction modification systems that protect bacteria and archaea against invading foreign DNA. Restriction endonucleases are the enzymes that recognise the specific palindromic sequences in the DNA and cleave the DNA strands at a particular restricted site. In recombinant DNA technology, these enzymes are used to cut the gene of interest and vector in which the foreign DNA is inserted. When make cut by using the same restriction enzyme for both foreign DNA and vector, the resultant DNA fragments have the same kind of 'sticky-ends' and, these can be joined together using DNA ligases. The recombinant vector molecule cannot be created when cuts are made by different enzymes.