EASY
AS and A Level
IMPORTANT
Earn 100
Explain the term sticky end.
Important Questions on Genetic Technology
MEDIUM
AS and A Level
IMPORTANT
Four different lengths of DNA, A to D, were cut with the restriction enzyme EcoRI. These fragments had the following numbers of restriction sites for this enzyme: A- 5, B- 7, C- 0, and D- 3. State the number of fragments that will be formed from each length of DNA after incubation with EcoRI.
HARD
AS and A Level
IMPORTANT
Describe the features of the restriction sites shown in the table below.
HARD
AS and A Level
IMPORTANT
Summarise the advantages of using plasmids as vectors in genetic engineering.
HARD
AS and A Level
IMPORTANT
Suggest why the primers used in PCR do not anneal together.
MEDIUM
AS and A Level
IMPORTANT
Explain why only certain DNA polymerases, such as Taq polymerase, are used in PCR.
HARD
AS and A Level
IMPORTANT
State what happens to DNA during one cycle in PCR.
HARD
AS and A Level
IMPORTANT
Suggest why the FBI uses STRs from the X and Y chromosomes in its database.
HARD
AS and A Level
IMPORTANT
Gene therapy for diseases such as ADA-SCID and LCA is possible because they are caused by recessive alleles. Explain how it might be possible to treat or cure Huntington's disease which is caused by a dominant allele.
