Match the following lists. List I List II A) Remove nucleotides from the end of the DNA I) Polymerases B) Restriction enzyme II) Endonuclease C) Enzyme which cuts at specific position of DNA III) Ligase D) Stick the complementary cut ends of DNA with H 2 bond IV) Nucleases V) Exonucleases The correct match is:

A = V , B = IV , C = II , D = III

A = V , B = I , C = III , D = II

A = III , B = II , C = IV , D = V

A = IV , B = I , C = II , D = III

H. Boyer & S. Cohen are famous for their research work on

Construction of the first artificial DNA molecule

Structure of double-stranded DNA

Discovery of restriction enzymes

Invention of Polymerase Chain Reaction

E M B I B E

EMBIBE CHAPTER WISE PREVIOUS YEAR PAPERS FOR BIOLOGY>Chapter 21 - Principles and Processes of Biotechnology>TS EAMCET Medical 2021 (9-Aug Shift-1)>Q 8

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IMPORTANT

Earn 100

Features of sparged stirred tank bioreactor that differ from simple stirred bioreactors
I) More bubble formation
II) Decrease the surface area for $O_{2}$ transfer
III) It has foam control system

50% studentsanswered this correctly

Important Questions on Principles and Processes of Biotechnology

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TS EAMCET

IMPORTANT

EMBIBE CHAPTER WISE PREVIOUS YEAR PAPERS FOR BIOLOGY>Chapter 21 - Principles and Processes of Biotechnology>TS EAMCET - 9 August 2021 Shift 2>Q 9

Which DNA fragment of a vector that helps in identifying and eliminating non-transformant cells and permit the growth of transformant cells?

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IMPORTANT

EMBIBE CHAPTER WISE PREVIOUS YEAR PAPERS FOR BIOLOGY>Chapter 21 - Principles and Processes of Biotechnology>TS EAMCET - 9 August 2021 Shift 2>Q 10

Assertion (A): Chromogenic substrates give blue-coloured colonies if the plasmid does not have an insert.

Reason (R): Recombinant colonies produce colour in presence of the chromogenic substrates due to the presence of $β$ galactosidase.
The correct option among the following is:

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IMPORTANT

EMBIBE CHAPTER WISE PREVIOUS YEAR PAPERS FOR BIOLOGY>Chapter 21 - Principles and Processes of Biotechnology>TS EAMCET - 10 August 2021 Shift 1>Q 11

Identify the correct statements.
(A) In gel electrophoresis, DNA fragments resolve according to their size.
(B) It is not necessary to digest the bacterial cells with lysozyme to isolate DNA
(C) In PCR, DNA polymerase extends the primers using the nucleotides.
(D) During the isolation of DNA, ribonuclease is added along with protease
(E) Agarose gel electrophoresis is employed to check the progression of restriction enzyme digestion.