Given figures represent the steps involved in Polymerase Chain Reaction ( $P C R$ ). Identify the steps $A$ , $B$ , $C$ and $D$ , ant select the correct option.

$A$	$B$	$C$	$D$
Denaturation at $94 - 96^{o}$ C	Extension through Taq polymerase at $72^{o}$ C	Repetition of denaturation and annealing	Annealing at $72^{o}$ C

$A$	$B$	$C$	$D$
Denaturation at $94 - 96^{o}$ C	Annealing at $40 - 60^{o}$ C	Extension through Taq polymerase at $72^{o}$ C	Repetition of denaturation and annealing

$A$	$B$	$C$	$D$
Denaturation at $40 - 60^{o}$ C	Annealing at $72^{o}$ C	Extension through Taq polymerase at $72^{o}$ C	Repetition of denaturation and annealing

$A$	$B$	$C$	$D$
Extension through Taq polymerase at $72^{o}$ C	Denaturation at $40^{o}$ C	Annealing at $72^{o}$ C	Repetition of denaturation and annealing

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Important Questions on Biotechnology: Principles and Processes

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IMPORTANT

MTG OBJECTIVE NCERT AT YOUR FINGERTIPS BIOLOGY CLASS XI + XII>Chapter 34 - Biotechnology: Principles and Processes>MCQS CORNER>Q 89

In a Polymerase Chain Reaction (PCR), after the denaturation step, why the mixture needs to cool down to a lower temperature?

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MTG OBJECTIVE NCERT AT YOUR FINGERTIPS BIOLOGY CLASS XI + XII>Chapter 34 - Biotechnology: Principles and Processes>MCQS CORNER>Q 90

If a recombinant DNA tearing gene for resistance to antibiotic ampicillin is transferred to E.coli cells, the host cells become transformed into ampicillin-resistant cells. If such bacteria are transferred on agar plates containing ampicillin, only transformants will grow and the untransformed recipient cells will die. The ampicillin-resistant gene in this case is called as

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MTG OBJECTIVE NCERT AT YOUR FINGERTIPS BIOLOGY CLASS XI + XII>Chapter 34 - Biotechnology: Principles and Processes>MCQS CORNER>Q 91

Which of the following is not used to transfer the recombinant DNA into the host?

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MTG OBJECTIVE NCERT AT YOUR FINGERTIPS BIOLOGY CLASS XI + XII>Chapter 34 - Biotechnology: Principles and Processes>MCQS CORNER>Q 92

A device in which large volume of living cells are cultured in order to get a specific product is called

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MTG OBJECTIVE NCERT AT YOUR FINGERTIPS BIOLOGY CLASS XI + XII>Chapter 34 - Biotechnology: Principles and Processes>MCQS CORNER>Q 93

Which of the following statements are correct with respect to a bioreactor?
(i) It can process large volumes of culture.
(ii) It provides optimum temperature and pH.
(iii) It is a completely automated tool.
(iv) It is a compact thermal cycler.

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MTG OBJECTIVE NCERT AT YOUR FINGERTIPS BIOLOGY CLASS XI + XII>Chapter 34 - Biotechnology: Principles and Processes>MCQS CORNER>Q 94

Identify the figures (A) and (B) and select the correct option:
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