How Humulin is produced?

Give the role of:

Tissue plasminogen activator (TPA)

Give the role of:

Tissue growth factor-Beta (TGF-$\beta$) in gene therapy.

With regard to insulin choose correct options.

(a) C-peptide is not present in mature insulin.

(b) The insulin produced by rDNA technology has C-peptide.

(c) The pro-insulin has C-peptide.

(d) A-peptide and B-peptide of insulin are interconnected by disulphide bridges.

Statements related to human Insulin are given below: Which statement(s) is/are correct about genetically engineered insulin?

(a) Pro-hormone insulin contains an extra stretch of C-peptide.

(b) A-peptide and B-peptide chains of insulin were produced separately in E. coli, extracted, and combined by creating disulphide bond between them.

(c) Insulin used for treating diabetes was extracted from cattle and pigs.

(d) Pro-hormone insulin needs to be processed for converting into a mature and functional hormone.

(e) Some patients develop allergic reactions to foreign insulin.

Choose the most appropriate answer from the options given below.

Assertion (A): Molecular fariming is large scale production of biochemicals from plants.

Reason (R) : Transgenic plants are bioreactors for commercial production of antibodies. 

The correct option among the following is:

Match the following 

The correct answer is

Identify the correct sequence of steps that are followed in gene therapy.
A Altered virus is mixed with cells from patient
B. Therapeutic human gene is inserted into the virus (vector)
C. Cells are collected from the patients
D. Cells from the patient become genetically altered
E. Reintroduction of genetically altered cells into patient
F. Suitable virus (vector) is selected and altered
G The genetically altered cells produce the desired proteins or hormones