MEDIUM
AS and A Level
IMPORTANT
Earn 100

How could you carry out an experiment to determine the effect of temperature on the rate of breakdown of hydrogen peroxide by catalase? 

Important Questions on Enzymes

MEDIUM
AS and A Level
IMPORTANT
Trypsin is a protease secreted in pancreatic juice, which acts in the duodenum. If you add trypsin to a suspension of milk powder in water, the enzyme digests the protein in the milk, so that the suspension becomes clear. How could you carry out an investigation into the effect of pH on the rate of activity of trypsin? (A suspension of 4g of milk powder in 100cm' of water will become clear in a few minutes if an equal volume of a 0.5% trypsin solution is added to it.) 
MEDIUM
AS and A Level
IMPORTANT

Sketch the shape that would be in the given graph, if excess hydrogen peroxide were not available. (Celery extract contains catalase enzyme).

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MEDIUM
AS and A Level
IMPORTANT
For each substrate concentration tested, the rate should be measured as soon as possible. Explain why. 
EASY
AS and A Level
IMPORTANT

Outline an investigation you could carry out to compare the temperature at which the enzyme lactase is completely denatured within ten minutes 

When free in solution 

MEDIUM
AS and A Level
IMPORTANT

Outline an investigation you could carry out to compare the temperature at which the enzyme lactase is completely denatured within ten minutes,

When immobilised in alginate beads

EASY
AS and A Level
IMPORTANT

Outline how you would determine the optimum pH of the enzyme lactase

When free in solution 

EASY
AS and A Level
IMPORTANT
The diagram shows an enzyme and two inhibitors of the enzyme, X and Y. Which of the following describes the two inhibitors? 
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EASY
AS and A Level
IMPORTANT
In a reaction controlled by an enzyme, which of the following graphs shows the effect of substrate concentration on the rate of the reaction, assuming the reaction is allowed to go to completion?