
In four separate experiments (Table), a vector and a fragment was digested with the indicated restriction enzymes. Both, the digested vector and were purified and ligated. represents the ampicillin resistant gene and Ori is the origin of replication. The resultant recombinant vector was transformed into Escherichia coli cells and incubated in a liquid nutrient medium with ampicillin for hours at . Based on the restriction enzymes used for cloning, choose the correct option.
Experiment No.
Restriction enzymes used
1
BamHI and SalI
2
EcoRI and SalI
3
KpnI and HindIII
4
KpnI and SalI


Important Questions on Biotechnology : Principles and Processes






Write short note on the following :
Gene gun











Assertion (A): Chromogenic substrates give blue-coloured colonies if the plasmid does not have an insert.
Reason (R): Recombinant colonies produce colour in presence of the chromogenic substrates due to the presence of galactosidase.
The correct option among the following is:



