MEDIUM
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In four separate experiments (Table), a vector DNA pBxyz6000bp and a DNA-X 1500 bp fragment was digested with the indicated restriction enzymes. Both, the digested vector and DNA-X were purified and ligated. Ampr represents the ampicillin resistant gene and Ori is the origin of replication. The resultant recombinant vector was transformed into Escherichia coli cells and incubated in a liquid nutrient medium with ampicillin for 24 hours at 37°C. Based on the restriction enzymes used for cloning, choose the correct option.

Experiment No. Restriction enzymes used
1 BamHI and SalI
2 EcoRI and SalI
3 KpnI and HindIII
4 KpnI and SalI

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Important Questions on Biotechnology : Principles and Processes

MEDIUM
Which among the following is a selectable marker in pBR – 322 ?
HARD
Give an account of the Blue-White Method of selection of recombinants.
HARD
By particles of which metal recombinant DNA is coated in particle bombardment?
MEDIUM
Explain any two methods for introduction of recombinant DNA. into host cell.
EASY
109 bacteria were spread on an agar plate containing penicillin. After incubation overnight at 37oC 10 bacterial colonies were observed on the plate. That the colonies are likely to be resistant to penicillin can be tested by
EASY
Write the methods to introduce alien DNA into Host cells.
EASY
In biotechnology applications, a selectable marker is incorporated in a plasmid
HARD
Describe two vector less methods of gene transfer used in rDNA technology.
EASY
A gene whose expression helps to identify transformed cell is known as
EASY
An antibiotic resistance gene in a vector usually helps in the selection of
EASY
The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of
MEDIUM

Assertion (A): Chromogenic substrates give blue-coloured colonies if the plasmid does not have an insert.

Reason (R): Recombinant colonies produce colour in presence of the chromogenic substrates due to the presence of β galactosidase.
The correct option among the following is:

EASY
In cloning vectors, antibiotic resistance genes are helpful for.
EASY
The technique of bombarding plant cells with high velocity microparticle's of gold or tungsten, coated with DNA, is