HARD
AS and A Level
IMPORTANT
Earn 100
Make a diagram to show how a piece of DNA is cut by restriction enzymes and then inserted into a plasmid. Show both strands of DNA in your diagram.
Important Questions on Genetic Technology
HARD
AS and A Level
IMPORTANT
Suggest why the primers used in PCR do not anneal together.
MEDIUM
AS and A Level
IMPORTANT
Explain why only certain DNA polymerases, such as Taq polymerase, are used in PCR.
HARD
AS and A Level
IMPORTANT
State what happens to DNA during one cycle in PCR.
HARD
AS and A Level
IMPORTANT
Suggest why the FBI uses STRs from the X and Y chromosomes in its database.
HARD
AS and A Level
IMPORTANT
Gene therapy for diseases such as ADA-SCID and LCA is possible because they are caused by recessive alleles. Explain how it might be possible to treat or cure Huntington's disease which is caused by a dominant allele.
HARD
AS and A Level
IMPORTANT
Summarise the challenges posed by gene therapy that have had to be overcome by researchers.
HARD
AS and A Level
IMPORTANT
What are the limits to gene therapy? Discuss the social and ethical issues surrounding this technology. Decide which procedures are ethical and which are not.
HARD
AS and A Level
IMPORTANT
Several crop species have been genetically modified to express poisons based on a toxic compound from the bacterium, B. thuringiensis (Bt). GM varieties of soya, oilseed rape, cotton, maize, and tobacco are all grown in countries such as the United States, China, and Brazil. Explain the advantages of growing Bt varieties of these crop plants.
