HARD
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Name the another name of gene guns method by which an alien DNA can be made to enter plant cell.

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Important Questions on Biotechnology - Principles and Processes

MEDIUM
Write down the functions of restriction enzyme and ligase enzyme. 
EASY
109 bacteria were spread on an agar plate containing penicillin. After incubation overnight at 37oC 10 bacterial colonies were observed on the plate. That the colonies are likely to be resistant to penicillin can be tested by
EASY
Which one of the following represents a palindromic sequence in DNA?
EASY
In biotechnology applications, a selectable marker is incorporated in a plasmid
MEDIUM

Assertion (A): Chromogenic substrates give blue-coloured colonies if the plasmid does not have an insert.

Reason (R): Recombinant colonies produce colour in presence of the chromogenic substrates due to the presence of β galactosidase.
The correct option among the following is:

EASY
The cutting of DNA at a specific location became possible with the discovery of:
EASY

Following statements describe the characteristics of restriction endonucleases. Identify the incorrect statement.

EASY
The technique of bombarding plant cells with high velocity microparticle's of gold or tungsten, coated with DNA, is
EASY
The DNA fragments separated on an agarose gel can be visualised after staining with:
EASY
In cloning vectors, antibiotic resistance genes are helpful for.
MEDIUM
Discuss the importance of cloning vectors in biotechnology.
HARD
Describe two vector less methods of gene transfer used in rDNA technology.
HARD
Give an account of the Blue-White Method of selection of recombinants.
EASY
A gene whose expression helps to identify transformed cell is known as
EASY
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using