EASY
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RNA interference takes place in all eukaryotic organisms as a method of cellular defence. This method involves silencing of a specific mRNA due to a molecule:

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Important Questions on Biotechnology and Its Applications

MEDIUM
r-DNA inserted within a coding sequence results in the process ________
EASY
Silencing of a gene could be achieved through the use of
HARD
Plasmid pBR322 has PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E. coli strain
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Assertion (A): All Eukaryotic cells have RNAi for cellular defence.

Reason (R): RNAi silences mRNA and prevents translation.

MEDIUM

Assertion (A): The fragments of DNA generated by the action of restriction endonucleases are separated by polyacrylamide gel electrophoresis.

Reason (R): The separated DNA fragments are visualised as bright orange coloured bands after staining with Ethidium Bromide followed by exposure to UV - radiation.

Which of the following is true?

MEDIUM

Study the following statement regarding rDNA technology and select the incorrect ones ?
(i) Taq polymerase extends the primers using the nucleotides provided in the reaction
(ii) Antibiotic resistance genes are considered as desirable genes in rDNA technology
(iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis
(iv) Transformation is a procedure through which a piece of DNA is introduced in a host bacterium
(v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture
(vi) Downstream processing is one of the steps of PCR

EASY
DNA fragments of 100 base pairs (bp), 300 bp and 500 bp were separated by agarose gel electrophoresis. Pick the correct arrangement of the fragments separated on the gel in the increasing order of their migration from the wells.
EASY
Name the nematode that infects the roots of tobacco plants? Name the strategy adopted to prevent this infestation.
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Silencing of a specific mRNA translation could be achieved through
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dsRNA is used to develop pest resistant tobacco plant by a technique called
HARD
How are DNA fragments visualised in Gel electrophoresis? What is elution?
EASY
In RNA interference, the dsRNA molecule prevents ___.
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How has the use of Agrobacterium as vectors helped in controlling Meloidogyne incognita infestation in tobacco plants? Explain in the correct sequence.
MEDIUM

Identify the labeled parts 1, 2, 3, 4 in the given figure?

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MEDIUM
Name the nematode that infects the roots of tobacco plants. Name the strategy adopted to prevent this infestation.
EASY

Identify the labels M and N in the following Agarose gel electrophoresis representation.

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EASY
What is the following is correct for the movement of DNA fragments on agarose gel during gel electrophoresis?