HARD
Earn 100
Read statements (I-IV) about recombinant DNA technology, and select the option that has only correct statement/s.
(I) E.coli cloning vector pBR322 have several restriction sites, ori, antibiotic resistance genes and rop.
(II) Competent bacterial cells can not take up vectors.
(III) In elution, the separated bands of RNA are extracted from the gel piece.
(IV) The downstream processing and quality control testing vary from product to product.
(II) Competent bacterial cells can not take up vectors.
(III) In elution, the separated bands of RNA are extracted from the gel piece.
(IV) The downstream processing and quality control testing vary from product to product.
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Important Questions on Principles and Processes of Biotechnology
EASY
The cutting of DNA at a specific location became possible with the discovery of:
EASY
Which of the following is a restriction endonuclease?
EASY
Which one of the following represents a palindromic sequence in DNA?
EASY
The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of
EASY
The DNA fragments separated on an agarose gel can be visualised after staining with:
EASY
Commonly used vectors for human genome sequencing are:
EASY
A gene whose expression helps to identify transformed cell is known as
EASY
In gel electrophoresis, separated DNA fragments can be visualized with the help of
EASY
What is the following is correct for the movement of DNA fragments on agarose gel during gel electrophoresis?
EASY
bacteria were spread on an agar plate containing penicillin. After incubation overnight at 10 bacterial colonies were observed on the plate. That the colonies are likely to be resistant to penicillin can be tested by
EASY
DNA's precipitation out of a mixture of biomolecules can be achieved by treatment with:
EASY
Restriction enzyme work by cleaving
EASY
Which vector can clone only a small fragment of DNA?
EASY
Following statements describe the characteristics of restriction endonucleases. Identify the incorrect statement.
EASY
Identify the palindromic sequence.
EASY
The specific palindromic sequence which is recognized by EcoRl is
MEDIUM
Identify the wrong statement with regard to restriction enzymes.
HARD
Eco and Rsa restriction endonucleases require 6bp and 4bp sequences respectively for cleavage. In a 10 kb DNA fragment how many probable cleavage sites are present for these enzymes
EASY
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
EASY
The introduction of T-DNA into plants involves :
