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Earn 100
Recombinant DNA technology can be used to produce quantities of biologically active form. Which one of the following products synthesized in E.coli
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Important Questions on Biotechnology - Principles and Processes
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Match the following:
| a | Origin of replication | i | Vector |
| b | Restriction enzymes | ii | gene amplification |
| c | Plasmid DNA | iii | initiation of replication |
| d | PCR | iv | molecular scissors |
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In recombinant DNA technology which of the following tools are required:
(a) restriction enzymes
(b) polymerase enzyme
(c) ligases
(d) vector
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The second two letters in the naming of restriction enzymes represent:
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Choose the correct statements:
(a) Each restriction endonuclease recognizes a specific palindromic sequence in the DNA.
(b) Restriction endonucleases are used in genetic engineering to form recombinant molecule of DNA.
(c) Fragments can be separated by a technique known as gel electrophoresis.
(d) Separated DNA fragments can be visualised by staining with ethidium bromide in visible light.
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The process for transfer the recombinant DNA into the host, cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA known as
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Arrange the following statements in particular order that how process of recombinant DNA technology takes place:
(a) isolation of desired DNA
(b) ligation of DNA fragment into a vector
(c) culturing the host cells in medium
(d) isolation of desired DNA fragment
(e) fragmentation of DNA by restriction enzyme
(f) extraction of desired product
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Thermostable enzyme which is utilised during amplification of DNA using PCR.
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At a time when the symptoms of the disease are not yet visible, very low concentration of a bacteria or virus can be detected by which technique -
