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Significance of treating bacterial cells with calcium chloride before transformation is to facilitate
(a)Binding of DNA to the cell surface
(b)Uptake of DNA through membrane transport proteins
(c)Uptake of DNA by creating transient pores in the bacterial cell wall
(d)Expression of antibiotic resistance gene

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Important Questions on Biotechnology - Principles and Processes
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Who among the following was awarded the Nobel Prize for the development of technique?

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Pure DNA precipitated by addition of chilled ethanol can be removed from solution by

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The optimum temperature for polymerisation in PCR is _____ while the enzyme responsible for the mentioned step can tolerate temperature up to _____. Select the correct option according to the blanks.

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Separation and purification by filtration, centrifugation of desired compound produced in bioreactor is a part of

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If a probe is made of radioactively labelled and is allowed to hybridise to its complementary in a cloned cell followed by detection using autoradiography. Which of the following is considered wrong for the given statements?

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Why would lac gene be fused to coding sequence of chain and in plasmids of E.coli?

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Melting of DNA at is due to breakdown of

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Plasmids are suitable vectors for gene cloning because they
