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Significance of treating bacterial cells with calcium chloride before transformation is to facilitate

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Important Questions on Biotechnology - Principles and Processes

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Who among the following was awarded the Nobel Prize for the development of PCR technique?
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Pure DNA precipitated by addition of chilled ethanol can be removed from solution by
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The optimum temperature for polymerisation in PCR is _____ while the enzyme responsible for the mentioned step can tolerate temperature up to _____. Select the correct option according to the blanks.
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Separation and purification by filtration, centrifugation of desired compound produced in bioreactor is a part of
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If a probe is made of radioactively labelled dsDNA and is allowed to hybridise to its complementary DNA in a cloned cell followed by detection using autoradiography. Which of the following is considered wrong for the given statements?
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Why would lac z gene be fused to coding sequence of chain A and B in plasmids of E.coli?
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Melting of DNA at 94°C is due to breakdown of
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Plasmids are suitable vectors for gene cloning because they