Some steps involved in the production of genetically engineered insulin are given below. Choose the correct sequence.

(i) Isolation of the insulin gene from a cell.

(ii) Culturing recombinant bacteria in the medium.

(iii) Insertion of the human insulin gene into a plasmid.

(iv) Introduction of the recombinant plasmid into bacteria.

(v) Production of active insulin.

(vi) Extraction of a recombinant gene product from the culture medium.

Figure given below depict the procedure for gene therapy. Pick up the disorders for which this technique has been applied successfully.

Read the following steps which are followed during the process of gene therapy while treating a patient of SCID. Arrange these steps in an order.
(i) Retrovirus infects lymphocytes extracted from bone marrow of the patient and cultured.
(ii) Engineered cells are injected into patient's bone marrow.
(iii) Normal allele is inserted into a retrovirus.
(iv) Retrovirus makes a DNA copy of its RNA. This DNA carrying the normal allele gets inserted into the chromosome of the host cell.
Arrange the above given steps in correct sequence and select the correct option.