Match the following techniques or instruments with their usage: (a) Bioreactor (i) Separation of DNA fragments (b) Electrophoresis (ii) Production of large quantities of products (c) PCR (iii) Detection of pathogen, based on antigen-antibody reaction (d) ELISA (iv) Amplification of nucleic acids Select the correct option from following:

( a ) - ( ii ) , ( b ) - ( i ) , ( c ) - ( iv ) , ( d ) - ( iii )

( a ) - ( iii ) , ( b ) - ( ii ) , ( c ) - ( iv ) , ( d ) - ( i )

( a ) - ( iv ) , ( b ) - ( iii ) , ( c ) - ( ii ) , ( d ) - ( i )

( a ) - ( ii ) , ( b ) - ( i ) , ( c ) - ( iii ) , ( d ) - ( iv )

EASY

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Important Questions on Biotechnology - Principles and Processes

MEDIUM

Biology>Biotechnology>Biotechnology - Principles and Processes>Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

Match the following techniques or instruments with their usage:

(a)	Bioreactor	(i)	Separation of DNA fragments
(b)	Electrophoresis	(ii)	Production of large quantities of products
(c)	$PCR$	(iii)	Detection of pathogen, based on antigen-antibody reaction
(d)	$ELISA$	(iv)	Amplification of nucleic acids

Select the correct option from following:

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

The Taq polymerase enzyme is obtained from:

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

To obtain reliable results from PCR machine, the process can be carried out for about ______ cycle.

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Biology>Biotechnology>Biotechnology - Principles and Processes>Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

HARD

Biology>Biotechnology>Biotechnology - Principles and Processes>Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

Which of the following is not a component of downstream processing?

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

Taq polymerase that finds its application in PCR is obtained from:

MEDIUM

Biology>Biotechnology>Biotechnology - Principles and Processes>Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

Which one among the following primer pairs would amplify the fragment of $DNA$ given below?

$5^{'} - CTAGTCGTCGAT - {(N)}_{300} - GACTGAGCTGAGCTG - 3'$

$3^{'} \cdot GATCAGCAGCTA - (N)_{300} - CTGACTCGACTCGAC - 5^{'}$

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

The two polypeptides of human insulin are linked together by:

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Biology>Biotechnology>Biotechnology - Principles and Processes>Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

Nowadays, the early diagnosis of bacterial or viral infection in humans is possible using

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Biology>Biotechnology>Biotechnology - Principles and Processes>Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

From the given combinations of steps in PCR, identify the enzyme dependent step/s.

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Biology>Biotechnology>Biotechnology - Principles and Processes>Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction)?

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Biology>Biotechnology>Biotechnology - Principles and Processes>Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

Stirred-tank bioreactors have been designed for:

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

Which of the following is not an application of PCR (Polymerase Chain Reaction)?

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

Rapid antigen test and RT-PCR are the two diagnostic tests for Covid

19

virus. PCR is a molecular diagnostic tool, that stands for:

HARD

Biology>Biotechnology>Biotechnology - Principles and Processes>Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

The correct order of steps in Polymerase Chain Reaction (PCR) is

HARD

Biology>Biotechnology>Biotechnology - Principles and Processes>Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

This entry in the table is wrongly matched, identify.

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

Name a peptide hormone which acts mainly on hepatocytes and adipocytes and enhances cellular glucose uptake and utilisation.

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Biology>Biotechnology>Biotechnology - Principles and Processes>Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

Suppose DNA samples collected for DNA fingerprinting analysis are less than the required quantity. Which of the following techniques is helpful to make the samples sufficient for above analysis?

MEDIUM

Biology>Biotechnology>Biotechnology - Principles and Processes>Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

Select the correct steps involved in amplification of gene

EASY

Biology>Biotechnology>Biotechnology - Principles and Processes>Process of Recombinant DNA Technology- Isolation of DNA and its Amplification

The process of separation and purification of expressed protein before marketing is called

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Important Questions on Biotechnology - Principles and Processes

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