HARD
AS and A Level
IMPORTANT
Earn 100
Suggest why the primers used in PCR do not anneal together.
Important Questions on Genetic Technology
MEDIUM
AS and A Level
IMPORTANT
State the four types of free nucleotides (dNTPs) that are added to reaction vessels at the start of the PCR process.
MEDIUM
AS and A Level
IMPORTANT
Explain why only certain DNA polymerases, such as Taq polymerase, are used in PCR.
HARD
AS and A Level
IMPORTANT
State what happens to DNA during one cycle in PCR.
HARD
AS and A Level
IMPORTANT
How many molecules of DNA are produced from one double-stranded starting molecule, after eight cycles of PCR?
HARD
AS and A Level
IMPORTANT
Explain why it is not possible to use PCR to increase the number of RNA molecules in the same way as it is used to increase the number of DNA molecules.
HARD
AS and A Level
IMPORTANT
Suggest why the FBI uses STRs from the X and Y chromosomes in its database.
HARD
AS and A Level
IMPORTANT
The latest estimate of the number of genes in the human genome is between 19000 and 22000. Before the invention of microarrays, it was very time-consuming to find out which genes were expressed in any particular cell. Explain how it is possible to find out which genes are active in a cell at a particular time in its development.
HARD
AS and A Level
IMPORTANT
The latest estimate of the number of genes in the human genome is between 19000 and 22000. Before the invention of microarrays, it was very time-consuming to find out which genes were expressed in any particular cell. Why is it not possible to use the same technique to find out which genes are active in red blood cells?
