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The chemical method of gene transfer is used in organisms like bacteria.

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Important Questions on Biotechnology - Principles and Processes

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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Write down the functions of restriction enzyme and ligase enzyme. 
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Following statements describe the characteristics of restriction endonucleases. Identify the incorrect statement.

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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Enzyme which joins two ends of DNA strand is 
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
DNA's precipitation out of a mixture of biomolecules can be achieved by treatment with:
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The DNA fragments separated on an agarose gel can be visualised after staining with:
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The introduction of T-DNA into plants involves :
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which vector can clone only a small fragment of DNA?
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
A gene whose expression helps to identify transformed cell is known as
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which one of the following represents a palindromic sequence in DNA?
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Sequence of strategies involved for the isolation of gene of interest from a plant cell during the process of rDNA technology
A) Dissolution of biological membranes
B) Enzymatic digestion of cell wall
C) Precipitation of DNA
D) Removal of DNA by spooling
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The cutting of DNA at a specific location became possible with the discovery of:
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The first step of genetic engineering is 
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Discuss the importance of cloning vectors in biotechnology.
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.

A. Insertion of recombinant DNA into the host cell.

B. Cutting of DNA at specific location by restriction enzyme.

C. Isolation of desired DNA fragment.

D. Amplification of gene of interest using PCR.

Choose the correct answer from the options given below: 

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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
In gel electrophoresis, separated DNA fragments can be visualized with the help of
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
What is the following is correct for the movement of DNA fragments on agarose gel during gel electrophoresis?
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Commonly used vectors for human genome sequencing are:
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which of the following is a restriction endonuclease?