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The restriction endonucleases are utilised in genetic engineering as
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Important Questions on Principles and Processes of Biotechnology
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The Taq polymerase enzyme is obtained from:
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The introduction of T-DNA into plants involves :
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What is the following is correct for the movement of DNA fragments on agarose gel during gel electrophoresis?
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Which of the following is a restriction endonuclease?
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The cutting of DNA at a specific location became possible with the discovery of:
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Eco and Rsa restriction endonucleases require 6bp and 4bp sequences respectively for cleavage. In a 10 kb DNA fragment how many probable cleavage sites are present for these enzymes
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The DNA fragments separated on an agarose gel can be visualised after staining with:
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Following statements describe the characteristics of restriction endonucleases. Identify the incorrect statement.
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The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of
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Which of the following is not a component of downstream processing?
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A gene whose expression helps to identify transformed cell is known as
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Stirred-tank bioreactors have been designed for:
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The process of separation and purification of expressed protein before marketing is called
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bacteria were spread on an agar plate containing penicillin. After incubation overnight at 10 bacterial colonies were observed on the plate. That the colonies are likely to be resistant to penicillin can be tested by
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Which one of the following represents a palindromic sequence in DNA?
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DNA's precipitation out of a mixture of biomolecules can be achieved by treatment with:
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Commonly used vectors for human genome sequencing are:
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A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
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Restriction enzyme work by cleaving
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Which of the following is not a feature of the plasmids?
