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The step of the mechanism of cloning includes

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Important Questions on Breeding and Biotechnology

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Diagrammatically represent the experimental steps in cloning and expressing a human gene (say the gene for growth hormone) into a bacterium like E. coli?
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The cloning of an animal for the first time was successfully performed by:
HARD
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Which of the following is/are used in recombinant DNA technology?
1. Agarose gel
2. Restriction endonuclease
3. Plasmid vector
4. Ethidium bromide
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We can cut the DNA with the help of 
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"Dolly” sheep was obtained by which method?
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The term 'recombinant DNA' refers to
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How is a probe used in molecular diagnostics?
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_____ technique made it possible to genetically engineer living organisms. (Gene cloning/ Gene therapy)
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Dolly was born on
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Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
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rDNA is a 
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The idea of recombinant DNA technique was first proposed by
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A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be:
HARD
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Write the steps you would suggest to be undertaken to obtain a foreign-gene-product.
HARD
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Which of the following are the basic steps of the gene cloning process?
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A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be
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Restriction endonucleases cut the DNA molecule at specific positions known as _____.
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1% of the DNA sequence that is a small stretch of repeated DNA sequences is called _____ DNA.
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Which of the following is not required in the preparation of a recombinant DNA molecule?
HARD
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Which of the following is used to produce products useful to humans by biotechnology techniques?