The trend of isoelectric point (pI) of the amino acids glycine, Iysine and aspartic acid is_____.

A nonapeptide in rat on hydrolysis gave the following identifiable tripeptides:
$\mathrm{Gly}-\mathrm{Ala}-\mathrm{Phe}, \mathrm{Ala}-\mathrm{Leu}-\mathrm{Val}, \mathrm{Gly}-\mathrm{Ala}-\mathrm{Leu}, \mathrm{Phe}-\mathrm{Glu}-\mathrm{His}, \text{and} \mathrm{His}-\mathrm{Gly}-\mathrm{Ala}.$

The sequence of the nonapeptide is