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What is common between EcoRI and HindII?

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Important Questions on Biotechnology - Principles and Processes

EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
HARD
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The correct order of steps in Polymerase Chain Reaction (PCR) is
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Restriction enzyme work by cleaving
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The DNA fragments separated on an agarose gel can be visualised after staining with:
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The Taq polymerase enzyme is obtained from:
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
What is the following is correct for the movement of DNA fragments on agarose gel during gel electrophoresis?
HARD
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which of the following is not a component of downstream processing?
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The process of separation and purification of expressed protein before marketing is called
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The introduction of T-DNA into plants involves :
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
A gene whose expression helps to identify transformed cell is known as
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which of the following is not a feature of the plasmids?
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
109 bacteria were spread on an agar plate containing penicillin. After incubation overnight at 37oC 10 bacterial colonies were observed on the plate. That the colonies are likely to be resistant to penicillin can be tested by
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Stirred-tank bioreactors have been designed for:
HARD
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Eco RI and Rsa I restriction endonucleases require 6bp and 4bp sequences respectively for cleavage. In a 10 kb DNA fragment how many probable cleavage sites are present for these enzymes
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Commonly used vectors for human genome sequencing are:
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which one of the following represents a palindromic sequence in DNA?
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The cutting of DNA at a specific location became possible with the discovery of:
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which of the following is a restriction endonuclease?
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?