MEDIUM
Earn 100

What is the definition of plasmid?

Important Questions on Breeding and Biotechnology

MEDIUM
Name a "natural genetic engineer" of plants. (Agrobacterium tumefaciens/Rhizobium/ Aspergillus)
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Which of the following is not required in the preparation of a recombinant DNA molecule?
EASY
Dolly, a sheep, was the first mammal to be cloned.
MEDIUM
Name a recombinant vaccine that is currently being used in vaccination program.
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Can you recall meiosis and indicate at what stage of prophase I, recombinant DNA is made? (Leptotene/ Zygotene/ Pachytene)
EASY
Who discovered the recombinant DNA technology?
MEDIUM
Diagrammatically represent the experimental steps in cloning and expressing a human gene (say the gene for growth hormone) into a bacterium like E. coli?
HARD
A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be
MEDIUM
A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be:
MEDIUM
When a foreign DNA is introduced into an organism, how is it maintained in the host and how is it transferred to the progeny of the organism?
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Explain the application of rDNA technology.
EASY
The cloning of an animal for the first time was successfully performed by:
MEDIUM
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
EASY
The term 'recombinant DNA' refers to
HARD
Write the steps you would suggest to be undertaken to obtain a foreign-gene-product.
HARD
Which of the following is/are used in recombinant DNA technology?
1. Agarose gel
2. Restriction endonuclease
3. Plasmid vector
4. Ethidium bromide
MEDIUM
How is a probe used in molecular diagnostics?
HARD
You have identified a useful gene in bacteria. Make a flowchart of the steps that you would follow to transfer this gene to a plant.