Enzymes

Hydrolytic enzymes which act on low pH are called as

Study the given data and answer the following question.

A sample of an enzyme called lactase was isolated from the intestinal lining of a calf. Assays were undertaken to evaluate the activity of the enzyme sample. The substrate of lactase is the disaccharide lactose. Lactase breaks a lactose molecule in two, producing a glucose molecule and a galactose molecule.

Two assays were carried out.

Assay 1:

Assay 2:

Which of the following statements can be concluded from the two assays?

A researcher determined the initial velocity $\left({\mathrm{V}}_0\right)$ of an enzyme in three different reactions (corresponding to lines $P,Q$ and $R$ in the graph) with increasing substrate concentrations $\left(\left[S\right]\right)$. The results were analyzed using a reciprocal plot where $1/V_0$ is plotted against $1/\left[S\right]$ (Graph). Reaction $1$ was performed without any inhibitory compound. Reaction $2$ was performed with compound-$X$ that inhibits enzyme activity by binding to the substrate binding site. Reaction $3$ was performed with compound $Y$ that inhibits enzyme activity by binding to a site other than the substrate binding site. If $K_m$ of the enzyme in reaction $1$ is $0.5\mathrm{mM}$, then the correct statement(s) from the following options is(are)

The quantities ${\mathrm{K}}_{\mathrm{m}}$ and ${\mathrm{V}}_{\max }$ were measured independently for an enzymatic reaction using enzymes $\mathrm{E}1, \mathrm{E}2, \mathrm{E}3$ and $\mathrm{E}4$ isolated from four different organisms (Table). For each enzymatic reaction, $0.5\mathrm{mM}$ substrate was used to obtain the product. The correct option in terms of product formation is

Height of a plant is positively determined by the amount of final product, $\mathrm{K}$, of a metabolic pathway catalyzed by five enzymes $\mathrm{L},\mathrm{N},\mathrm{P},\mathrm{M}$ and $\mathrm{O}$ (as depicted below). The intermediate product, $\mathrm{I}$, blocks the activity of the enzyme $\mathrm{P}$, whereas intermediate $\mathrm{J}$ blocks the enzyme $\mathrm{L}$. Conversion of $\mathrm{I}$ to $\mathrm{J}$ is the rate limiting step. Based on this information, choose the correct statement from below.

A scientist performed enzyme kinetics and obtained velocity$\mathrm{v}$ as a function of substrate concentration $\left[\mathrm{S}\right]$ and the values are given in the table below.

The ${\mathrm{K}}_{\mathrm{m}}$ of the substrate could

The graph given below shows the Michaelis-Menten kinetics for two reactions ($R_1$ and $R_2$; represented as solid and dotted lines, respectively). Enzymes $E_1$ and $E_2$ have $V_{\max }$ values, $V_{\max 1}$ and $V_{\max 2}$, respectively. The respective $K_M$ values for $E_1$ and $E_2$ are $K_{M1}$ and $K_{M2}$. ($K_{M1}=K_{M2}$).

The correct statement(s) for reactions $R_1$ and $R_2$ is/are

Enzyme concentration has no effects on the rate of a chemical reaction.

List some commercial applications of enzymes.

Define allosteric enzyme.

Give one example of negative feedback example?

Describe the three components of negative feedback inhibition?

What is negative feedback inhibition?

What is Isoenzymes?

Write the enzyme action of catalytic cycle?

Difference between enzyme activity and specific activity?

What is enzyme concentration?

Select the correct option:
Assertion: All proteinaceous enzymes have a three-dimensional structure.
Reason: The secondary structure of a protein is according to amino acids present inside the polypeptide.   

Match the entries in column I and Column II. Which one of the following choices is correct?

Dehydrogenase are mainly responsible for oxidation of its substrates whereas reductase are mainly responsible for reduction of its substrates.