Replication
Replication: Overview
This topic covers concepts such as DNA Replication, DNA Polymerase, Replication Fork, DNA Ligase, Origin of Replication, Leading and Lagging Strands, Okazaki Fragments, Semi Discontinuous Replication, etc.
Important Questions on Replication
Which of the following enzyme plays role in only the formation of discontinuous strand of DNA?

Which of the following chemical substance is used as a source of nitrogen in the Meselson and Stahl’s experiment?

Curling of tendrils is due to

What is the error in above diagram?

Restriction endonucleases are used as:

Write short note on the following
Origin of replication

Name the enzyme which is used to join DNA fragments, whether it is endonuclease or ligase.

DNA replication is a discontinuous process.

Meselson and Stahl used isotopes of which element?

Which one of the following will be the ratio of heavy, intermediate and light bands in Meselson and Stahl's experiment after two generations if replication were conservative?

Which one of the following elements is not directly involved in transcription?

Role of E.coli in replication of DNA is
i. Semi-conservative replication was proved first
ii. Polymerises approximately per second
iii. Replication time is minutes
iv. Length of DNA is
The correct answer is:

With respect to DNA synthesis, identify the correct combination of statements.
I. Always the direction of DNA polymerization is when referring to the polarity of strand being synthesized.
II. DNA ligase forms hydrogen bonds between two newly synthesized DNA strands.
III. DNA polymerase on its own cannot initiate the process of replication.
IV. DNA polymerase can catalyze polymerization in both and directions.

Which one of the following statements is correct?


The experiment on DNA using isotope proved that its replication is _____.

Explain the process of DNA replication.

What is the function of an RNA primer during DNA replication?

Why is DNA replication called semiconservative?

Given below are various enzymes used in DNA replication. Select the correct match of enzyme with its function.
