Replication

IMPORTANT

Replication: Overview

This topic covers concepts such as DNA Replication, DNA Polymerase, Replication Fork, DNA Ligase, Origin of Replication, Leading and Lagging Strands, Okazaki Fragments, Semi Discontinuous Replication, etc.

Important Questions on Replication

EASY
IMPORTANT

Which of the following enzyme plays role in only the formation of discontinuous strand of DNA?

MEDIUM
IMPORTANT

Which of the following chemical substance is used as a source of nitrogen in the Meselson and Stahl’s experiment?

MEDIUM
IMPORTANT

Curling of tendrils is due to

EASY
IMPORTANT

Question Image

What is the error in above diagram?

EASY
IMPORTANT

Restriction endonucleases are used as:

MEDIUM
IMPORTANT

Write short note on the following

Origin of replication

EASY
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Name the enzyme which is used to join DNA fragments, whether it is endonuclease or ligase.

EASY
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DNA replication is a discontinuous process.

EASY
IMPORTANT

Meselson and Stahl used isotopes of which element?

MEDIUM
IMPORTANT

Which one of the following will be the ratio of heavy, intermediate and light bands in Meselson and Stahl's experiment after two generations if DNA replication were conservative?

EASY
IMPORTANT

Which one of the following elements is not directly involved in transcription?

MEDIUM
IMPORTANT

Role of E.coli in replication of DNA is
i. Semi-conservative replication was proved first
ii. Polymerises approximately 2000 bp per second
iii. Replication time is 38 minutes
iv. Length of DNA is 6.6×109bp

The correct answer is:

MEDIUM
IMPORTANT

With respect to DNA synthesis, identify the correct combination of statements.

I. Always the direction of DNA polymerization is 5'3' when referring to the polarity of strand being synthesized.

II. DNA ligase forms hydrogen bonds between two newly synthesized DNA strands.

III. DNA polymerase on its own cannot initiate the process of replication.

IV. DNA polymerase can catalyze polymerization in both 5'3' and 3'5' directions.

MEDIUM
IMPORTANT

Which one of the following statements is correct?

EASY
IMPORTANT

DNA strand separation enzyme

MEDIUM
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The experiment on DNA using N15 isotope proved that its replication is _____.

HARD
IMPORTANT

Explain the process of DNA replication.

MEDIUM
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What is the function of an RNA primer during DNA replication?

MEDIUM
IMPORTANT

Why is DNA replication called semiconservative? 

MEDIUM
IMPORTANT

Given below are various enzymes used in DNA replication. Select the correct match of enzyme with its function.