Transcription

IMPORTANT

Transcription: Overview

This topic covers concepts, such as Transcription of DNA, Transcription Unit, Template Strand, Coding Strand, Promoter sequence, Structural Gene, Terminator sequence, Monocistronic Gene, Polycistronic Gene, Exons, Introns, etc.

Important Questions on Transcription

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IMPORTANT

During transcription, the template strand is the one with the polarity:

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IMPORTANT

TATA box of eukaryotic promotor lies

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If coding strand of DNA has the following sequence, find the last codon to 5'- ATG CCT TTC TAG -3' coding strand incorporate amino acid in mRNA.

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In eukaryotes, RNA polymerase III is responsible for synthesis of:

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In the process of transcription, the strand of DNA with polarity _____ acts as a _____ strand.

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The transcription of any gene is the indication of its

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In a transcription unit in a DNA segment, a promoter is located

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Select the correct statement:

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The base sequence of mRNA is similar to ........................ except for that uracil will be present in place of thymine in mRNA.

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Which ONE of the following polymerases transcribes 5S rRNA?

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Which type of RNA polymerase enzyme transcribes precursor mRNA?

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Which type of RNA polymerase enzyme transcribes precursor mRNA? 

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Find out the series of genes in the transcription unit of the following statements.

I. Located towards 3 '-end of the structural gene.

II.  Flanked by the promoter and the terminator.

III. Located towards 3'-end of the coding strand.

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Match the following

List-I List - II List -III
 A)  tailing  i) 28 s,18 s,5.8 s I) Transported out of nucleus
 B)  r-RNA  ii) Addition of methyl
guanosine triphosphate
II) 3 'end
C) Caping iii) m-RNA III) Transcribes by RNA polymerase
D) Processed
hn RNA
iv) Addition of Adenylate
Residue
IV) 5 ' end of hn RNA

The correct answer is

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Assertion (A) : During transcription both the strands of DNA are copied

Reason (R):   If both strands act as template DNA would code for RNA molecule with different sequences

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Identify the correct statement:

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Assertion (A): The structural gene in a transcription unit can be said to be monocistronic or polycistronic.

Reason (R): In eukaryotes, the monocistronic structural gene has interrupted coding sequence - but the genes in eukaryotes split. The coding sequence or expressed sequences are defined as exons. Exons are said to be those sequence that appears in mature or processed RNA.
The correct answer is

MEDIUM
IMPORTANT

What is the corresponding sequence of mRNA when a polynucleotide template DNA strand is 3'-TAC GGT AAG CGG ATC-5'?

HARD
IMPORTANT

Read the following statements and choose the correct option.
(i) Tailing: It is addition of adenylate residues at 5' -end in a template independent manner.
(ii) Promoter: It is the site recognised by the RNA polymerase in association with ρ factor.
(iii) Division of labour: RNA polymerase I and RNA polymerase III are responsible for 18S rRNA and tRNA transcription respectively.
(iv) Capping: addition of methyl guanosine triphosphate at the 5'-end of hnRNA.

EASY
IMPORTANT

If the template strand of DNA is 3'ATGCATGCATGCATGC 5', identify the sequence of RNA transcribed.