Replication

IMPORTANT

Replication: Overview

This Topic covers sub-topics such as DNA Replication, DNA Polymerase, Okazaki Fragments, Meselson and Stahl's Experiment, Replication Fork, DNA Ligase, Leading and Lagging Strands and, Origin of Replication

Important Questions on Replication

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IMPORTANT

Choose the enzyme that is involved in the continuous replication of DNA strand.

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Which of the following enzyme plays role in only the formation of discontinuous strand of DNA?

MEDIUM
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Which of the following chemical substance is used as a source of nitrogen in the Meselson and Stahl’s experiment?

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Curling of tendrils is due to

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Question Image

What is the error in above diagram?

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Restriction endonucleases are used as:

HARD
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A DNA molecule having labelled thymidine in one of its strands is allowed to replicate in a radioactive medium. Calculate the number of strands with labelled thymine in the DNA after three successive divisions.

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The addition of nucleotide during DNA replication is done by:

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Meselson and Stahl used isotopes of which element?

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Which one of the following will be the ratio of heavy, intermediate and light bands in Meselson and Stahl's experiment after two generations if DNA replication were conservative?

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Which one of the following elements is not directly involved in transcription?

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Role of E.coli in replication of DNA is
i. Semi-conservative replication was proved first
ii. Polymerises approximately 2000 bp per second
iii. Replication time is 38 minutes
iv. Length of DNA is 6.6×109bp

The correct answer is:

MEDIUM
IMPORTANT

With respect to DNA synthesis, identify the correct combination of statements.

I. Always the direction of DNA polymerization is 5'3' when referring to the polarity of strand being synthesized.

II. DNA ligase forms hydrogen bonds between two newly synthesized DNA strands.

III. DNA polymerase on its own cannot initiate the process of replication.

IV. DNA polymerase can catalyze polymerization in both 5'3' and 3'5' directions.

MEDIUM
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Which one of the following statements is correct?

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DNA strand separation enzyme

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At the time of DNA replication template with polarity 5'3', new strand synthesised:-

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Okazaki fragments are :-

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DNA polymerase is required for the synthesis of

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How many times can DNA replicate in a life of a cell?

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θ (theta) replication of DNA is observed in which of the following?