Classification of Enzymes

IMPORTANT

Classification of Enzymes: Overview

This Topic covers sub-topics such as Enzymes, Activation Energy, Enzyme Inhibition, Coenzyme, Cofactors, Ribozyme, Competitive Inhibition, Apoenzymes, Enzyme Structure, Prosthetic Group, Nomenclature of Enzymes, Holoenzymes and, Active Site of Enzymes

Important Questions on Classification of Enzymes

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Hydrolytic enzymes which act on low pH are called as

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The class of enzyme that catalyzes the reaction is 

S - G + S’ → S + S’ - G

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What is ribozyme enzyme and how is it an exception?

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Which of the following is correct with respect to the of the enzyme on the equilibrium position?

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What is protease enzyme?

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What is a holoemzyme?

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Study the given data and answer the following question.

A sample of an enzyme called lactase was isolated from the intestinal lining of a calf. Assays were undertaken to evaluate the activity of the enzyme sample. The substrate of lactase is the disaccharide lactose. Lactase breaks a lactose molecule in two, producing a glucose molecule and a galactose molecule.

Two assays were carried out.

Assay 1:

Lactose concentration (% w/v) 15 15 15 15 15 15
Concentration of enzyme sample (% v/v) 0 5 10 15 20 25
Reaction rate μmol glucose sec-1 mL-1 0 25 50 75 100 125

Assay 2:

Lactose concentration (% w/v) 0 5 15 20 25 30
Concentration of enzyme sample (% v/v) 5 5 5 5 5 5
Reaction rate μmol glucose sec-1 mL-1 0 15 25 35 40 40

Which of the following statements can be concluded from the two assays?

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With reference to enzymes, turnover number means

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During enzymatic reactions, enzyme-substrate complex is:

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The complete enzyme is called:

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Select the correct option:
Assertion: All proteinaceous enzymes have a three-dimensional structure.
Reason: The secondary structure of a protein is according to amino acids present inside the polypeptide.   

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Match the entries in column I and Column II. Which one of the following choices is correct?

  Column I   Column II
(a) Haeme (1) Cofactor
(b) NAD (2) Vitamin
(c) Zinc (3) Coenzyme
(d) Niacin (4) Prosthetic group

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Which group of enzymes catalyses reactions involving a structural rearrangement of a molecule?

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Which among the following enzymes, help in joining the molecules together?

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Which one has more free energy?

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If an enzyme reaction follows the Michaelis Menten kinetics represented by the equation:

V0=VmaxSKM+S

Where V0 is the initial reaction velocity, Vmax is the maximal reaction velocity,  KM is the Michaelis constant, and [S] is the substrate concentration. Which of the following statements is correct?

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Enzymes functional inside the cells are called:

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Esterase belongs to the category of:

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Protein nature of enzyme was first discovered by:

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Which of the following is a cofactor of carboxypeptidase, a proteolytic enzyme?